You are studying the function of the Darth Vader protein (DV) in a Drosophila cell culture model.

| April 14, 2018

You are studying the function of the Darth Vader protein (DV) in a Drosophila cell culture model. After passaging your cells a few times, you notice a small subpopulation has emerged that expresses DV at ~70% of wild-type levels. You sequence the Darth Vader coding sequence and promoter (~400 nt upstream of coding sequence) and find no mutations. You name this subpopulation ROTJ cells. As a clever undergraduate student, you think DV is being silenced by RNAi in ROTJ cells so you knock-out Dicer … 

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