Which population of vesicles has a surface similar to that of the cell

| April 14, 2018

Homework Assignment #41.Your friend has isolated plasma membranes and reassembled the membranes intosmall vesicles. Using fluorescently labeled lectin, he sees that some of his vesiclesare fluorescently labeled and some are not. Recall that lectin binds tocarbohydrates. Furthermore, his labeled lectin cannot permeate membranes.A.Which population of vesicles has a surface similar to that of the cell?Why?B.How do you explain the other population of vesicles?2.You have isolated a bacterial transporter that transports Mg2+ and Ca2+ in oppositedirections. You produce lipid vesicles that contain this Mg2+–Ca2+ pump as thesole membrane protein. The Mg2+–Ca2+ pumps are oriented such that Mg2+ ispumped into the vesicle while Ca2+ is pumped out. Each pump transports oneMg2+ ion into the vesicle and one Ca2+ ion out of the vesicle during each pumpingcycle and ATP hydrolysis powers this transport, as illustrated in Figure below.A.Predict what would happen if the solution inside the vesicles contained 75mM Mg2+ and 75 mM Ca2+ ions, while the solution outside the vesiclescontained 75 mM Mg2+ and 75 mM Ca2+ ions as well as 1 mM ATP, andyou had a 10,000 fold excess of fluid outside the vesicles compared withB.the volume inside the vesicles. Specifically, be sure to state whether youthink there would be net movement of ions and if so, how theconcentrations of the Mg2+ and Ca2+ ions inside and outside the bilayerwould change. In addition, be sure to state whether you would be creatingan electrical gradient, a chemical gradient, or both.Explain how long the pump will run.

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