The opportunity to propose a molecular assay

| August 31, 2017

Question
we have been given the opportunity to propose a molecular assay and develop a molecular laboratory. This will require that we write a proposal to administration. This proposal must include our assay design and equipment list. we must propose how we would use the available space given the included floor plan. Each section of this exercise will require that we adhere to the space limitations.

1. ONE PAGE MAXIMUM

The gene we will need to design a test around is provided. The polymorphism is noted:

5′

301 gtcagcccca tggtggtggc tggggacagc cacatggtgg tggaggctgg ggtcaaggtg

361 gtagccacag tcagtggaac aagcccagta agccaaaaac caacaygaag catgtggcag

421 gagctgctgc agctggagca gtggtagggg gccttggtgg ctacatgctg ggaagtgcca

3′

481 tgagcaggcc tcttatacat tttggcaatg actatgagga ccgttactat cgtgaaaaca

Describe in detail the assay we will design. Be as specific as possible. No make believe enzymes or reagents. Nothing magical.

At a minimum our description should include:

-A diagram indicating where any primers or probes would anneal to

the above sequence (we can annotate the sequence above).

-A list of any reagents needed, i.e. oligonucleotides, enzymes, etc.

-A statement of how we will detect the polymorphisms

-Controls needed

-The concentrations and formulations of any gels used

-The concentrations and components of any enzymatic reactions used

-A diagram of how the results of our assay would appear including

controls, a homozygous individual and a heterozygote.

Priniciple of test:

Reagents needed:

Controls needed:

Outline of assay:

Diagram of results:

2. ONE PAGE MAXIMUM

Given the following floor plan, indicate how we would assign the activities related to our assay. describe how we would control contamination. At a minimum it is expected that we will include areas for:

Specimen processing

Data Analysis

Pre-PCR

Post-PCR

Reagent preparation

Storage

Darkroom

Show where refrigerators, freezers, thermocyclers, safety equipment, hoods and other equipment.

indicate traffic flow if it is integral to our contamination control plan.

we are not allowed to add, move or remove walls. we can add doors if we feel it necessary

1/2″ = 5 ft

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