The following data were obtained for an enzyme-catalyzed reaction in the absence ofinhibitor and with inhibitor A or inhibitor

| February 14, 2018

The following data were obtained for an enzyme-catalyzed reaction in the absence of

inhibitor and with inhibitor A or inhibitor B. In each case, the inhibitor is present a10 μM.

[S] (mM) v (uninhibited) v (inhibitor A) v(inhibitor B)

0.333 1.65 1.05 0.794

0.400 1.86 1.21 0.893

0.500 2.13 1.43 1.020

0.666 2.49 1.74 1.190

1.000 2.99 2.22 1.43

2.000 3.72 3.08 1.79

From the data above, using the direct linear plot (it is Eisenthal-Cornish-Bowden direct

plots), determine:

A. km

B. vmax C. The type of inhibition by A.

D. The type of inhibition by B.

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