The design and validation of a diagnostic PCR assay for detection of UPEC

| December 28, 2015

The design and validation of a diagnostic PCR assay for detection of UPEC

Please read the instruction carefully
Production of a scientific paper on the design and validation of a diagnostic PCR assay for detection of UPEC. You will write the paper as indicated in the instructions to authors for the Journal of Clinical Microbiology
(https://jcm.asm.org/site/misc/journal-ita_org.xhtml#02)

The paper should introduce the concept of wishing to detect UPEC in urine samples, and the problems associated with this. You should then provide methods as appropriate for a journal article in this journal. You should then provide concise and relevant results from your bioinformatics analysis through to your primer design and the results of your PCR tests to validate the assay.

You are strongly encouraged to look at similar types of J Clin Microbiol articles to gauge how much detail and how many figures tables you should be adding to your paper. The article is worth 60% of the module mark and will be assessed as follows:

Abstract – as per authors instructions, concise and informative 5%

Introduction – fully introduces background and rationale of the research 15%

Methods – concise, relevant and in appropriate style 10%

Results – Concise relevant and well presented, with appropriate supporting commentary, avoiding addition of numerous irrelevant tables and figures 45%

Discussion – discuss your results in light of recent literature 20%

References – relevant, recent references in correct style format 5%

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