BIO 230 For Lab Exam II Isolation: Various methods of isolation, their features

| August 14, 2017

BIO 230 For Lab Exam II

Isolation: Various methods of isolation, their features, pros and cons,
and the appropriate use of each method. Know how to perform serial
dilutions and calculate bacterial concentration in a given sample. Know
the related calculations thoroughly.
Be able to use the calculations and/or techniques of isolation in an experiment.
Understand the technique of steak plate isolation, and how to perform it.
Capsule staining,
Acid-fast staining, endospore staining: Understand
the principle of each type of differential staining. Know the procedures
and trouble shootings. If asked, you should be able to perform it
successfully from a given a culture. Be able to recognize a particular
staining if a pre-stained slide is provided.
Control of bacterial
growth:Know the mode of action for each of
the chemicals tested in the lab and how they can be used on microorganisms
of specific characteristics. Be able to design an experiment to evaluate a
chemical’s effectiveness as an antimicrobial. Be able to evaluate the
effectiveness if a set of data from an experiment is provided.

UV: Understand the
mechanism of UV damage. How it can be used for bacterial control. Know how to
test the effectiveness of UV against microbes and factors that may interfere in
its antimicrobial activity.

Conjugation: Know how bacterial conjugation works and the outcome of the
process. Be able to apply the technique in testing genetic material
transfer in bacteria and analyze results from experiments similar to the
one conducted in class.

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